dotnet webapp not running on localhost:5000 / 50001 – Asp.net

I had the same problem like you.

Probably you have the problems with certificate.

So try to run this PowerShell script for that ports :

Start-Transcript -Path "$($MyInvocation.MyCommand.Path).log"
try {
    Write-Host "Creating cert resources"
    $ekuOidCollection = [System.Security.Cryptography.OidCollection]::new();
    $ekuOidCollection.Add([System.Security.Cryptography.Oid]::new("1.3.6.1.5.5.7.3.1","Server Authentication")) | Out-Null
    $sanBuilder = [System.Security.Cryptography.X509Certificates.SubjectAlternativeNameBuilder]::new();
    $sanBuilder.AddDnsName("localhost") | Out-Null

Write-Host "Creating cert extensions"
$certificateExtensions = @(
    # Subject Alternative Name
    $sanBuilder.Build($true),        
    # ASP.NET Core OID
    [System.Security.Cryptography.X509Certificates.X509Extension]::new(
        "1.3.6.1.4.1.311.84.1.1",
        [System.Text.Encoding]::ASCII.GetBytes("IIS Express Development Certificate"),
        $false),
        # KeyUsage
        [System.Security.Cryptography.X509Certificates.X509KeyUsageExtension]::new(
            [System.Security.Cryptography.X509Certificates.X509KeyUsageFlags]::KeyEncipherment,
            $true),
            # Enhanced key usage
    [System.Security.Cryptography.X509Certificates.X509EnhancedKeyUsageExtension]::new(
        $ekuOidCollection,
        $true),
        # Basic constraints
        [System.Security.Cryptography.X509Certificates.X509BasicConstraintsExtension]::new($false,$false,0,$true)
    )
Write-Host "Creating cert parameters"
$parameters = @{
    Subject = "localhost";
    KeyAlgorithm = "RSA";
    KeyLength = 2048;
    CertStoreLocation = "Cert:LocalMachineMy";
    KeyExportPolicy = "Exportable";
    NotBefore = Get-Date;
    NotAfter = (Get-Date).AddYears(1);
    HashAlgorithm = "SHA256";
    Extension = $certificateExtensions;
    SuppressOid = @("2.5.29.14");
    FriendlyName = "IIS Express Development Certificate"
}
Write-Host "Creating cert"
$cert = New-SelfSignedCertificate @parameters

$rootStore = New-Object System.Security.Cryptography.X509Certificates.X509Store -ArgumentList Root, LocalMachine
$rootStore.Open("MaxAllowed")
$rootStore.Add($cert)
$rootStore.Close()

Write-Host "Creating port bindings"
# Add an Http.Sys binding for port 44300-44399
$command = 'netsh'
for ($i=5000; $i -le 5002; $i++) {
    $optionsDelete = @('http', 'delete', 'sslcert', "ipport=0.0.0.0:$i")
    $optionsAdd = @('http', 'add', 'sslcert', "ipport=0.0.0.0:$i", "certhash=$($cert.Thumbprint)", 'appid={214124cd-d05b-4309-9af9-9caa44b2b74a}')
    Write-Host "Running $command $optionsDelete"
    & $command $optionsDelete
    Write-Host "Running $command $optionsAdd"
    & $command $optionsAdd
} 
}
catch {
    Write-Error $_.Exception.Message
}
finally {
    Stop-Transcript
}

I hope that will work for you too!